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Alternatively, the stock culture organisms could be developed in an appropriate liquid medium (i.e., SoybeanCasein Digest Broth or Sabouraud Dextrose Broth) as well as cells harvested by centrifugation, then washed and resuspended in sterile saline TS to acquire a microbial count of about 1 × 108 cfu per mL. [NoticeThe estimate of inoc